Journal of Basic and Clinical Reproductive Sciences

Journal of Basic and Clinical Reproductive Sciences
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Human Embryo Culture with Mineral Oils: A comparative Analysis of Oily Embryo Culture Incubation

Author(s): Noureddine Louanjli, Aya Al-Ibraheemi, Mohammed Zarqaoui, Mohamed Ennaji, Wassym R Senhaji, Ritu S Santwani, Romaissa Boutiche, Mustafa Zakaria

The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil™, a paraffin oil, and LiteOil, a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing In Vitro Fertilization (IVF) treatment by Intracytoplasmic Sperm Injection (ICSI) were enrolled in our study. After ICSI, 1237 oocytes were 1:1 randomly allocated into 2 groups according to the type of overlaying oil: Ovoil™ (616 oocytes) or LiteOil (621 oocytes). Fertilization rate was assessed around 18 hours post-insemination (hpi) and embryos were checked for early cleavage at 25 hpi. Embryo morphology was recorded on days 2 and 3. A total of 437 and 438 day 3 embryos were analyzed. There were no differences between the two groups in terms of fertilization rate and occurrence of early cleavage. The proportion of top-quality embryos (41.7% vs. 41.2%) and the final utilization rates (92.2% vs. 92.0%) were similar in Ovoil and LiteOil groups, respectively, at day 3. Live birth rate per transfer was essentially the same with Ovoil™ overlay (26.9%) when compared to LiteOil (26.2%). Live birth rate in patients who simultaneously received embryos from both overlay types was 17.2%. Despite the different characteristics of these two oils regarding hydrocarbon saturation, packing and temperature storage, Ovoil and LiteOil can be used in parallel in the same IVF protocol.

Select your language of interest to view the total content in your interested language

20+ Million Readerbase
Google Scholar citation report
Citations : 1261

Journal of Basic and Clinical Reproductive Sciences received 1261 citations as per google scholar report

Journal of Basic and Clinical Reproductive Sciences peer review process verified at publons
Indexed In
  • Google Scholar
  • Publons
  • Euro Pub
Submit your Manuscript

  • Flexible online submission
  • Preliminary quality report within 3 days
  • 21 days rapid peer-review
  • High visibility of published content
  • Associated with relevant global conferences
  • 24/7 Editorial office availability