This research article has been made to demonstrate the oocytes activation process and how it's related to Ca2+ oscillation. Moreover, the research shows how Ca2+ oscillation is transition pattern can be affected by the sperm types. It has been found that the oocysts activation depends on intercellular factors and single transduction which is mainly activated by Ca2+ oscillation. Ca2+ oscillation induction can have a different pattern in different spices, but it depends chiefly on one factor which the sperm-oocytes fusion. Through fertilization, the spermatozoon produces a series of low- long-lasting Ca2+ oscillations frequency. It is usually believed that these oscillations are caused by to Ca2+ release into the Inositol 1,4,5-Trisphosphate (InsP3) receptor. Thus, the Ca2+ oscillation can also relay on the type of sperm injected to the oocytes where it has been found that round spermatid is not as effective as spermatozoon to induce Ca2+ oscillation. Although round spermatid can be used in cases such as severe azoospermia, where no spermatozoon is available, with the help of artificial oocytes activation.